Journal: Microbial Cell Factories

Article Title: Ribosome engineering of Myxococcus xanthus for enhancing the heterologous production of epothilones

doi: 10.1186/s12934-024-02627-3

Figure Lengend Snippet: Fermentation of ZE9N-R22 in bioreactors. a DO curve in 3 L fermenter. b pH curve in 3 L fermenter. c Yields of epothilones in flasks and fermenters. d DO curve in 10 L fermenter. e pH curve in 10 L fermenter. f Biomass detection in 10 L fermenter. The red dotted line indicated the feeding time nodes

Article Snippet: For fed-batch fermentation in 10 L bioreactor (BioFlo 310, Eppendorf NBS, Germany) containing 5 L of CMO medium, nitrogen nutrition (casitone 150 g/L and yeast extract 75 g/L) and methyl oleate were added at feed rated of 140 ml /L and 6 ml/L, respectively.

Techniques:

Journal: Biotechnology for biofuels and bioproducts

Article Title: Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.

doi: 10.1186/s13068-023-02367-3

Figure Lengend Snippet: Fig. 8 Pulsed fed-batch cultivation of C. glutamicum ΔfasR cg2692TTG CgLP12::(Ptac-pntAB-TrrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate. Fed-batch cultivations were conducted in a 1-L BioFlo120® bioreactor system with an initial batch volume of 0.5 L in CgXIImod medium supplemented with 195 µM PCA. The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L−1. 30 mL pulses (P) of a 350 g glucose L−1 hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Shown process data are representative of three bioreactor cultivations using the same process conditions. Act acetate, DO dissolved oxygen, Glc glucose, X biomass, Xyl xylose

Article Snippet: Fed-batch cultivations were conducted in a 1 L BioFlo120® bioreactor system (Eppendorf SE, Hamburg, Germany) with an initial volume of 0.5 L. The cultivations were performed at 30 °C using an adjusted version of the CgXII medium to obtain high biomass concentrations (CgXIImod).

Techniques: Concentration Assay

Journal: Membranes

Article Title: The Effect of Heat Sterilization on Key Filtration Performance Parameters of a Commercial Polymeric (PVDF) Hollow-Fiber Ultrafiltration Membrane

doi: 10.3390/membranes12080725

Figure Lengend Snippet: A summary of hybrid biotechnological/membrane separation processes and the applied sterilization method. The dashes denote absence of relevant data.

Article Snippet: The handmade membrane modules were designed to be suitable for the head-plate and the height of a 3 L lab-scale bioreactor (BioFlo 120, Eppendorf, Hamburg, Germany).

Techniques: Membrane, In Situ, Serial Time-encoded Amplified Microscopy

Journal: Journal of biotechnology

Article Title: 3D printed autoclavable biocompatible biodegradable bioreactor vessels with integrated sparger made from poly-lactic acid.

doi: 10.1016/j.jbiotec.2024.06.001

Figure Lengend Snippet: Fig. 1. A) Simple bioreactor designed to be used in the Dasgip® Parallel Bioreactor System. B) Bioreactor vessel with the integrated zero dead-volume harvest line and sparger at the bottom of the reactor vessel. C) Detailed cut-out view of the integrated features on the bottom of the vessel.

Article Snippet: Batch cultivations were performed in a 1.5 L glass bioreactor with an L-sparger with a hole diameter of 0.5 mm (DS1500ODSS, 77102054, Eppendorf, Hamburg, Germany) and 3D printed bioreactor vessels with an integrated sparger with a hole diameter of 1.2 in the digital file and 0.8 measured in the final part.

Techniques:

Journal: Journal of biotechnology

Article Title: 3D printed autoclavable biocompatible biodegradable bioreactor vessels with integrated sparger made from poly-lactic acid.

doi: 10.1016/j.jbiotec.2024.06.001

Figure Lengend Snippet: Fig. 4. Bioreactor setup with the fully assembled 3D printed autoclaved bioreactor vessel (left) and glass vessel (right) set in place in the Das gip® Bioblock.

Article Snippet: Batch cultivations were performed in a 1.5 L glass bioreactor with an L-sparger with a hole diameter of 0.5 mm (DS1500ODSS, 77102054, Eppendorf, Hamburg, Germany) and 3D printed bioreactor vessels with an integrated sparger with a hole diameter of 1.2 in the digital file and 0.8 measured in the final part.

Techniques: